Antibody Repertoire High - Throughput Sequencing of the Zebrafish
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چکیده
www.sciencemag.org (this information is current as of May 7, 2009 ): The following resources related to this article are available online at http://www.sciencemag.org/cgi/content/full/324/5928/807 version of this article at: including high-resolution figures, can be found in the online Updated information and services, http://www.sciencemag.org/cgi/content/full/324/5928/807/DC1 can be found at: Supporting Online Material http://www.sciencemag.org/cgi/content/full/324/5928/807#otherarticles , 2 of which can be accessed for free: cites 12 articles This article http://www.sciencemag.org/cgi/collection/immunology Immunology : subject collections This article appears in the following http://www.sciencemag.org/about/permissions.dtl in whole or in part can be found at: this article permission to reproduce of this article or about obtaining reprints Information about obtaining
منابع مشابه
High-throughput sequencing of the zebrafish antibody repertoire.
Despite tremendous progress in understanding the nature of the immune system, the full diversity of an organism's antibody repertoire is unknown. We used high-throughput sequencing of the variable domain of the antibody heavy chain from 14 zebrafish to analyze VDJ usage and antibody sequence. Zebrafish were found to use between 50 and 86% of all possible VDJ combinations and shared a similar fr...
متن کاملDeterminism and stochasticity during maturation of the zebrafish antibody repertoire.
It is thought that the adaptive immune system of immature organisms follows a more deterministic program of antibody creation than is found in adults. We used high-throughput sequencing to characterize the diversifying antibody repertoire in zebrafish over five developmental time points. We found that the immune system begins in a highly stereotyped state with preferential use of a small number...
متن کاملWeinstein , Antibody Repertoire High - Throughput Sequencing of the Zebrafish
www.sciencemag.org (this information is current as of May 8, 2009 ): The following resources related to this article are available online at http://www.sciencemag.org/cgi/content/full/324/5928/807 version of this article at: including high-resolution figures, can be found in the online Updated information and services, http://www.sciencemag.org/cgi/content/full/324/5928/807/DC1 can be found at:...
متن کاملComprehensive Evaluation and Optimization of Amplicon Library Preparation Methods for High-Throughput Antibody Sequencing
High-throughput sequencing (HTS) of antibody repertoire libraries has become a powerful tool in the field of systems immunology. However, numerous sources of bias in HTS workflows may affect the obtained antibody repertoire data. A crucial step in antibody library preparation is the addition of short platform-specific nucleotide adapter sequences. As of yet, the impact of the method of adapter ...
متن کاملCharacterization of the naive murine antibody repertoire using unamplified high-throughput sequencing
Antibody specificity and diversity are generated through the enzymatic splicing of genomic gene segments within each B cell. Antibodies are heterodimers of heavy- and light-chains encoded on separate loci. We studied the antibody repertoire from pooled, splenic tissue of unimmunized, adult female C57BL/6J mice, using high-throughput sequencing (HTS) without amplification of antibody transcripts...
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تاریخ انتشار 2009